In the new version of cell profiler (v1.0.4628) there seems to be a problem when identifying primary objects. When I set “method to distinguish clumped objects or Method to draw dividing lines between clumped objects” to anything other than intensity the pipeline crashes. How can I fix this?
I will look in to this, other users have also brought up this bug. It is not immediately clear what is wrong. Sorry for the inconvenience!
Thanks for the reply. I get around this by using an older version of the software.