I would like to ask for some help and advice on making measurements from images. The white in images is gases flowing into and out of a model of the airway. There are 3 areas where I would like to measure degree of ‘brightness’ and ‘heterogeneity’ (how much light and dark) within each area.
I have made standard image selection areas (see below)
Here are three examples of how the images may look like depending on how much gas within the model airway:
I have used the median and standard deviation of brightness to quantify the amount of “white” in the different selected area:
I have a total of 1200 images which makes for 3600 set of measurements. I would like a way of having:
count, mean, stdDev, min, max and mode, as well as the raw histogram (which I get from pressing copy) in a spreadsheet, ideally, one sheet per frame with three sets of measurements per sheet and most ideally, image filename also put within the spreadsheet. Would be great to be able to batch process as doing this 3600 times manually is not an exciting thought.
Is there a way of doing this and anyone who may be willing to help? Also, do you think the way I am looking at brightness and mixing (mixture of grey and white means mixing of gas) the best variables to use?