Brighfield annotations with Dapi

Dear all,

Do you have any suggestions to automatically annotate BF glial cells (channel 1) using Dapi nuclei (channel 2 ) as a starting point? The idea is to create a DL model to automatically segment these cells without fluorescence.
example-3

Here is a sample in tif:

example-1.tif (11.5 MB)

Thank you in advance.

Mafalda

2 Likes

Hey Mafalda @mmvpgs ,

maybe the label-free prediction model by Ounkomol et al does that job or is at least a good starting point. It’s available as #zerocostdl4mic notebook:

Let us know if this helps!

Best,
Robert