I am using photos of wing membranes, back-lit with UV light.
I am looking to see how much of the wing fluoresces orange. What ratio of fluorescence is on the wing/total wing area.
- In previous protocol, they split up the image into red, green, and blue channels and then traced the sections manually with a table. Is splitting the image into channels possible to do without converting the image to gray scale?
- Are there better pipelines out there that I could use to determine fluorescent area/total wing area?