BAS_0067.tiff (625.2 KB)
Hi, all! I am new to Cellprofiler (I use version 3.1.9) and I am trying to extract features from blood smear images. I found a similar topic, but it was not successfully resolved.
Now I am trying to identify the nucleus and cells. However, the images are not fluorescent and the resolution is really not very high.
Many white blood cells have a bilobed or segmented nucleus. I used both inversion with IdentifyPrimaryObjects and UnmixColors with Watershed for this, but the cytoplasm is still very poorly and strangely determined by IdentifySecondaryObjects (I’ve tried to decrease noise too). Attached is the best I’ve achieved so far.
I need any ideas of how to:
- make the program not dividing complex nuclei into several parts
- deal with cell segmentation against the background of erythrocytes
PS: does it make sense to try Ilastik or ImageJ?
here is my pipelineblood_smears.cpproj (89.9 KB)