Blastocyst Segmentation

Hi all, I am trying to run a very similar analysis as that described in the cellProfiler 3.0 paper: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2005970 I am hoping to get more details on what exactly was done in figure 2A to achieve the segmentation. I understand the steps laid out, but a bit more detail on the parameters used (especially for the EorSFeatures step).

Thanks!

Hi @mervl,

EnhanceSuppressFeatures module is to enhance the signal from the background. In this pipeline too, the purpose is to enhance the nuclei signal. In case you need more details, you may have to elaborate the query.

Regards,
Lakshmi
www.wakoautomation.com