I am having an issue when I open my time-lapse images on Fiji through bioformats importer. The first image stack opens up fine and looks pretty much how it looked when I took the image on our scope. Every single spot beyond that opens and visually looks about 2/3 dimmer. I am not sure if this is just a visual bug and the pixel intensity values are actually more representative of what I see on our scope or if the images are actually different somehow (which would be a huge problem as I am quantifying intensity of a fluorescent reporter). Have any of you heard of this issue before? I am not opening the files correctly? Any suggestions or insight would be greatly appreciated.