I have an issue with the Bio-Format plug-in. I want to use it to open the output file of the Leica slide scanner, which is *.scn. Apparently, this is a “pyramidal tiff”. Please forgive me, if I’m technically incorrect.
Anyway, when importing the scn file, there is a couple of so-called “series” which I can select for import. Besides a couple of overview images that are saved within this file, there is several images of the area that was scanned in detail. These are probably the “pyramid”. I can open these up to the second highest resolution (which is 11264x16192). However, and this is the problem: I can not open the highest resolution image (45056x64768).
Opening the second highest image takes a couple of minutes, between 4 and 5. I would image that a picture with 16 times the amount of pixels would then take significantly longer. I have been waiting for 2hrs. Nothing happens. The activity monitor (btw, I’m on a mac) does not show any activity of Fiji (it does use a lot of computing power when opening the lower resolution image).
I have until tomorrow evening 21:30 to get an image out of this file that can be printed on DIN A4 with 300dpi. If I won’t, there goes the journal title page with my histology staining on it. So any help is very highly appreciated!