I am trying to align two .tif files generated at different times but on the same section in order to determine colocalisation of signal. I am trying to use bigwarp to do this. I open both the .tif files and start the plug in. However, there is an issue with one of the tif files. When I select it as the fixed image, it does not display within the dialogue box and when I select it as the moving image, I get the error below:
(Fiji Is Just) ImageJ 2.0.0-rc-67/1.52h; Java 1.8.0_172 [64-bit]; Windows 10 10.0; 8556MB of 12166MB (70%) java.lang.ArrayIndexOutOfBoundsException: 7 at bigwarp.BigWarpInit.namesFromImagePluses(BigWarpInit.java:513) at bigwarp.BigWarpInit.createBigWarpDataFromImages(BigWarpInit.java:406) at bdv.ij.BigWarpImagePlusPlugIn.run(BigWarpImagePlusPlugIn.java:79) at ij.IJ.runUserPlugIn(IJ.java:228) at ij.IJ.runPlugIn(IJ.java:192) at ij.Executer.runCommand(Executer.java:137) at ij.Executer.run(Executer.java:66) at java.lang.Thread.run(Thread.java:748)
Could anyone offer some advice on how to fix/troubleshoot please? I have updated imagej, tried using a smaller version of the tif file and am able to transform other images. I’m new to image analysis/manipulation so obvious/baisic suggestions welcome! Thanks in advance.