I have used the BigStitcher plugin in FIJI to stitch together a lightsheet image of an embryo. While it did stitch the images, there are some issues: 1) The individual tiles don’t always overlap well/isn’t smooth 2) There are striations throughout the data. Here is a link to show everyone what I mean. Embryo image
I’m assuming I would need to adjust some parameters, but not sure what I would need to adjust.