Does anyone have any experience/comments on using the BigStitcher plugin for multimodal registration and stitching? The end goal is quantitative image analysis by grabbing data from overlapping regions. BigStitcher was recommended to me as a good tool for stitching intra-modality, but if I understand correctly it may be capable of doing multimodal as well, and might be easier than integrating external registration tools
I’m quite new to the plugin and I have just begun playing around with it for regular intra-modal stitching, so I would appreciate any advice or suggestions about this topic. I’m also trying to automate the process through scripting.
For reference, here’s a graphic of what I’m trying to do. Boxes are the images I’m taking. The colored circles are fiducials for registration. Top left is the lowest resolution method (~50 micron voxels). The orange/green/blue boxes are singular tiles. Middle and left are the same resolution (~1 micron) and use the same optics, so should be aligned just by reading stage position. The black and red boxes are regular grids of many tiles.
Th original idea was registering left to middle, and by doing so registering it to the right and getting the sample data (transitively).