My aim with this pipeline is to obtain outlines and measurements for the entire cell. My issues are these:
The cell interior is speckled, so there isn’t a clear primary object with reference to which the secondary object can be outlined. To resolve this, I’ve tried a number of smoothing modules such as median/gaussian filters, threshold and smooth. This removes noise inside the cell body but fails to preserve the edges. I haven’t been successful with feature enhancing modules, such as circle-enhancing in the SuppressOrEnhanceFeatures module, or EnhanceEdges.
I’ve attached the pipeline I’ve been working with. Eager for advice about how to better capture cell outlines in this image.
Project.cpproj (640.0 KB)