I have a quick question for those who have experience working with high-resolution biological images. I am currently working on a project that requires me to use skeletonize 3D on a stack of about 2000 8-bit images that are 2560 x 2568 pixels in height and width and I cannot seem to process them without using up all the available RAM on my computer and having to stop the program. The easiest solution to this problem seems to be scaling the images so that they use less RAM. However, I’m not sure of the best way to reduce the size of these images without losing information. The Resize plugin seems to be fairly good at this as it can resize the images to about 1/2 the original size without losing much information at all. However, it still seems like this might be too large to process. Does anyone here have any suggestions? I’m fairly new to fiji and don’t know all the applications that might be available to help with procedures like this one. Any help would be great. If you have any questions that I can clarify, please let me know, thank you!