I am a PhD student from Germany.
I want to use cell profiler analyst for counting nuclear foci.
I have a limited knowledge of computers and am a biologist. I am using MAC laptop with Safari.
I downloaded from
cellprofiler.org/downloadCPA.shtml and copied it to the applications folder
Then I downloaded the pipeline below. It has three files (2 TIFF and one examples)
Speckle Counting: This pipeline shows how to identify smaller objects (foci) within larger objects (nuclei) and how to use the Relate module to establish a relationship between the two as well as perform per-object aggregate measurements (such as number of foci per nucleus).
[Download] (3 MB)
I then tried to open TIFF images with the CP analyst program from the Applications folder.
I receive the following reply
CPanalyst quit unexpectedly.
PLease help me to proceed further. Please note I am a beginner