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  • If you create a new topic, please attach to your post:
  • A description of the phenotype of interest, i.e, the cellular image features that you are looking for. Not all of your readers will be fluent in the details of your biological system or assay. A basic, novice-level description goes a long way to making sure that others understand your goals.
  • At least one sample image (or set of images that comprise a minimal data set). Better yet, an example of both a positive and a negative control.
  • A CellProfiler pipeline (i.e. a *.cppipe file). At least make an attempt!
  • Note that a CellProfiler Project file (*.cpproj) contains an image list, however unless they point to world-accessible files (e.g. URLs) then you will also need to attach the image sets separately.

Please help me to make a pipeline
Count number of GFAP+ cells (costained with DAPI)
Step wise guidance
Overlaying primary, secondary, and tertiary objects together
Identifying and coundting double-labeled cells
Problem opening images from properties file
Nuclear translocation help
How to run a cpproj file from terminal (Unix)?
Adjusting Brightness/Contrast
Number of LC3 dots per cell
Please help me with the CP pipelines
Is there manual crop module?
Maximum Intensity Projection
Endocytic organelles
Welcome to the CellProfiler forum!
H&E cell counting
Adipocyte pipeline- Identification problem
Cells distribution inside wells
Measuring Cell Fluorescence
Identification of myonuclei
Cannie Kidney and gliosarcoma
Saving images using metadata info for names
Nuclei, cytoplasm and cell counting
SEM images of Collagen Fibers
Measurement of female sexual cells
Pipeline for Wheat germ agglutinin staining
Cell-cell fusion pipeline or suggested modules?
Area of Tumor Spheroids
Property file opening
Help with segmentation of cells and pixel count
How can I exclude primary objects that are not centered by nuclei?
Tissue DAPI channel quantification
Determination of quantity of spherical particles
MCEC Fluorescein Diacetate and Propidium Iodide Staining
Identifying and coundting double-labeled cells
Counting dots on white paper
Need help with input module setting
CellProfiler not distinguishing between small droplets