I am new here and have no experience with programming. I just want to create a macro to set threshold values and create masks in the specified channels in my images. I figured it would run something like this:
- Open my images
- Split channels
- set threshold in channel 1 (100, 255)
- set threshold in channel 2 (100, 255)
I am not sure how complicated it is to split the channels and then select the correct channels via a macro. Also my microscope images are LIF. files, so I suppose I should convert them to TIF. directories first.
Please let me know if there something like this around already that I can play around with.