I’m a newbie and not a programmer so working with macros is pretty tough for me. In a cell experiment measured the fluorescence of 6 groups of cells t DAPI, 647, 568 and 488. I imaged these over 11 timepoints. In summary, I took images of 12 groups at timepoints every 3 mins for about 30 mins using 4 different channels. What I want to do is apply the same processing to each group for all timepoints (ie brightness correction etc in each channel), then quantify for each group/ timepoint the 488 fluorescence intensities in the ROIs and compare between timepoints (this should be possible as we use the same acquisition parameters). Since I need the processing to be the same for this analysis I guess I need some macro script to do it for me.
Would anyone be able to help me!