I heard about cell profiler at the ASCB meeting in December. I am very excited about it and have now started using it. I have a couple of questions, very basic.
I remember that there is a way to quantitate the cell size, and although I have been able to get images that represent the cells, I am unable to get a data sheet that shows a numerical readout of the cell size.
Is there a way to edit the image after it has been produced. With my current settings the cells are correctly identified in about 90% of the cases, but I would like to be able to correct the other 10% after the image is created (or better yet figure the settings out so that it works more accurately).
I have also been having problems in that the settings that correctly identify cells for some of my images are different than those that correctly identify cells for some of my other images. This results from some variation in morphology between cells. Is this something that can be componsated for?
(and this may sound silly) but I have the oddest problems opening cellprofiler. I click on the computer screen button looking button, which opens a black window and then somehow eventually, usually after some random clicking on other icons, the nice looking blue screen with the cellprofiler logo opens up and I can start working.
Thanks very much for any help you can provide with these matters.