Basic cell counting

Hello everyone

I’m very new to the use of CellProfiler but I would like to use it to count the number of cells in a picture taken from a confluent cell culture. The pictures are not very high in contrast unfortunately… I will add an example. I’ve tried various ways and settings but I feel like I am missing the right modules to be able to count them all, not just the very obvious ones. Many thanks for any tips/pipelines to try out!!

Hi,

This is unfortunately quite tricky. Please see this FAQ on brightfield images (they are brightfield, no? Any stains?):
cellprofiler.org/forum/viewtopic … 900e#p4488

You might also try the module UnmixColors to try and get the best grayscale separation of the colors here.

David