I recently started using CellProfiler, and I could really use any help or advice
with my problem.
I’m working on images like the ones I attached. I need to segment the axons in the images
(the black filaments) and then take some measurements (length, number of branches,…) on
them. I tried inverting the images and using the IdentifyPrimaryObjects
module for the segmentation, but its not working.
Do you have any suggestions?
Thanks for your time