Average pixels with line tool

I am using straight line selection tool to measure the fluorescent intensity of a cell. My cells vary in width so each measurement provides me with different number of data points. Is there a way to normalize how many data points I get with each line I draw regardless of the cell size?


Can you describe a bit more clearly what it is you wish to measure and how? Instead of drawing a line across each cell - can you segment the entire cell region and measure the intensity within and then normalize for area? Also - would you be able to post an example image that shows what you are trying to measure?


Hi @loren,

A script like this one can help you. It straightens the line and then interpolates the values you need and finally outputs the profile results onto a Results Window.

Hope this helps