Longest Branch pipeline .cppipe (13.5 KB)
I am currently using this pipeline to analyze adult NSC morphology/cytoskeleton structure. I’ve worked on creating mask images in hopes of obtaining a measurement for average neurite length of all neurites per cell or each individual neurite length or a longest neurite branch length per cell. My experimental condition is a dosage response curve. The increasing concentration is exhibiting less neurite branching, disruption of normal cytoskeleton extensions, and overall what seems to be a smaller measurement for all neurite length per cell.
I am also having difficulty with CellProfiler correctly identifying the nuclei of each cell. I will attach an image below to show my issue especially when two neurites from the same cell branch off from one another at junction and then come together.
Currently, the program seems to be more accurate at measuring individual cells, but I would love to be able to run multiple images through a pipeline that consisted of fields of view with multiple cells.