Average dye spread distance and fluorescence area(SL/DT) analysis via ImageJ?

Hello, everyone. I am performing scrape loading/dye transfer assay at moment(using lucifer yellow) and get some green fluorescent images for analyzing the Gap Junctional Intercellular Communication. The morphology of fluorescent area in images is irregular. Therefore don’t know how to calculate the average dye spread distance and average fluorescence area via imageJ. Is there anyone who could give me some advices? Thanks so much.

Hello Zizi, it is hard for people to help you without any example images to see what you are working with. Different images, different experiments, different analysis solutions. Maybe you can share a few of your images here?

Sverre

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I have solved this problem. Thanks all the same.