Automatic Width Detection

[Ferry_Saputra]

Hi everyone.

I have some problem with measuring the width of some blood vessel in imageJ.
I have this image from Z projection of some video and I want to automatically detect the size of the horizontal white line and the average width of the line from the most left until the most right part.
Is there any macro or plugin to do it?

Thank you.

[MatthieuV (Matthieu Vermeren)]

Hi Ferry,

There is this brilliant macro which you can adapt:

ImageJ macro to measure distance between two lines/edges

Here is an ImageJ macro to measure distance between two user-drawn segmented lines (polylines) on an image. Shortest distances from points along the shorter line to the longer line are used to calculate the average distance between the two lines (their spline interpolations). The macro works in ImageJ 2.1.0/1.53c, and may be useful to someone. The macro prompts user to draw the two segmented lines (example below). [Drawing lines] The macro then makes the measurements and displays statistics…

Beware though: you are using a Z projection. If your vessel twists a bit, the projection will make it look thicker than it really is.

Sincerely,

Matthieu

[Ferry_Saputra]

Hi Matthieu.

Thank you for the reply.
I already tried your macro and its work.
Although it still semi-automatic because we still need to draw straight line but this is already good enough and this macro can be used without the needed to change into z projection image.

Sincerely,

Ferry

[biovoxxel (Biovoxxel (Jan Brocher))]

The approach mentioned in that post might help. But you need first to threshold / segment your vessel.

How to calculate width of worms in the image?

Hi @amaze, another possibility would be to use the Local Thickness plugin (→Analyze→Local Thickness) in Fiji Since your provided image is already binary for the analysis you cound add after your segmentation the following analysis: run("Set Measurements...", "area mean standard modal min center perimeter fit shape feret's integrated median skewness kurtosis display redirect=None decimal=3"); run("Invert"); run("Analyze Particles...", "exclude clear add"); run("Local Thickness (masked, calibra…

[Ferry_Saputra]

Hi Jan.

I already tried it and it works well as long as the threshold value can be predetermined.
Thank you.