Thanks in advance for any help.
I’m having trouble doing something that seems simple, but apparently isn’t.
Basically, I’d like to know how to automatically identify the positions along a line where a given threshold occurs.
Images I’m working with tend to look like this:
The points I’d like to identify occur along the yellow line.
I think there are several different approaches that could work, but am missing the key piece, which is how to get imageJ to output the coordinates of the threshold points along the line.
The images could be binarized/thresholded:
It may also be possible to get the crossing points from a profile plot along the line:
My goal is to write a macro that will automate the analysis and data output for sets of images. The rest of the macro seems pretty straightforward, but the missing piece is how to automatically identify the positions along the line where the threshold occurs.
Suggestions greatly appreciated…