I’m working on segmentation in c elegans embryos. The dataset looks something like this: https://commons.wikimedia.org/wiki/File:Imaging-multicellular-specimens-with-real-time-optimized-tiling-light-sheet-selective-plane-ncomms11088-s7.ogv
I basically want to mark the cell membrane. I’ve tried several types of thresholding and morphological operations. Thresholding usually results in discontented cell boundaries. Morphological(using MorphoLibJ) operations usually results in over-segmentation or under-segmentation. I tried Trainable Weka Segmentation also[Problem: discontented cell boundaries].
The major problem is discontinuous cell boundaries in results.
Can anyone suggest some methods to achieve the required segmentation?
PS: The final aim is to track each cell (to get to know about their position vector, velocity vector, shape changes etc).