Hello there,I am a PhD student who is having a lot of problems trying to automate cell counting and measuring from wheat endosperm images. I have used a nice stain that outlines the cells nicely when transformed to a binary image. However I cannot for the life of me configure the analyze particles tool correctly so that it is just the dark areas within the lighter borders that are counted and measured rather than the cell walls themselves. In addition when I try to use the find edge tool fill tool from the binary menu I still don’t have much luck. I have posted the original image and any help or advice would be most appreciated.