Hi everyone, first of all I would like to wish you a happy Christmas.
For my graduation project needs, I am trying to write a python script in order automate the identification and the selection of the region of interest in a fluorescent microscopy image.
The identification of a region of interest can be done following several criteria, and that is what I am trying to identify first, as I am not a biologist or an expert microscopist I have no idea about the more used methods of criteria to track a region of interest.
I created this topic, in order to collect tips and advices from experimented fluorescent microscopy user or scientist that used to identify a region of interrest in a sample.
If anyone have any information that can help me, such as idea of criteria to track ( size, brightness intensity, shape, density of object…) Or anything else (papers, library,Codes…) I would be very grateful if you let me know…
Thank you !!