Auto focus error - Micro-manager

Hi,

I’m trying to perform autofocusing using a Point Grey Flea camera and Zaber z stage. I’m currently trying to use the JAF (H&P) plugin. I’m setting the parameters of the plugin as follows, however I’m not sure what should go in the Channel tab or what that means.

image

This is the error message I get when I press the autofocus now button on the main window.

Autofocus started.
Before rough search: 18900.362250000002
Error in device “Flea3 FL3-U3-120S3C_21013706”: No buffer arrived within the specified timeout. (10001)
Error in device “Flea3 FL3-U3-120S3C_21013706”: Camera not ready for software trigger (12301)
Error in device “Flea3 FL3-U3-120S3C_21013706”: Camera not ready for software trigger (12301)
Error in device “Flea3 FL3-U3-120S3C_21013706”: Camera not ready for software trigger (12301)
Error in device “Flea3 FL3-U3-120S3C_21013706”: Camera not ready for software trigger (12301)
BEST_DIST_SECOND18892.36125 BEST_SH_SECOND2.5650980426E10
Error in device “Flea3 FL3-U3-120S3C_21013706”: Camera not ready for software trigger (12301)
Total Time: 7812

I can hear the stage moving. Any help would be much appreciated on how to fix this issue.

Thanks,
Chaitanya

I tested autofocusing on MM 1.4. The OughtaFocus plugin seems to work for me.

This plugin was not in the list in MM v2, although the .jar file was in the MM plugins folder. How do I add this plugin to MM v2? Thanks in advance.

There is a problem in the build of the Oughtafocus plugin. I am researching the issue, but it turns out to be complicated. Hopefully will be solved soon.

Nick Anthony just pushed a fix for the build issue. The plugin should work again in tonight’s build. Thanks for reporting!

1 Like

Thanks again, Nico. I will test it with tonight’s build.

If its not too much trouble, can you help me understand the error I’m seeing with the JAF plugin? I’m unsure about the value that needs to go into the JAF(H&P) channel property.