I would like to count positive and negative cells for different manually drawn tissue regions.
In the example screenshot below I have positive and negative cells imported as two types of point objects (coming form an external image analysis workflow). I further have two tissue regions as polygon annotations. How can I assign positive and negative points by overlap to the two regions, to eventually count positives and negatives for each region? Preferably without scripting.
I tried by splitting the point clouds to single objects which assigns the point objects automatically, but then I lose their properties (name and color) to distinguish between positive and negative points.
(the displayed histology image is just a placeholder)
all the best