I’m using a 2-stack image where the FITC stain within each nucleus corresponds to the amount of damage within that nucleus. I have tried entering a published code for the macro (see pdf file after references), but the language is confusing and though I’m almost there, I’m still far away. Essentially, the point of the code is to outline each individual nuclei and then attain the area of the FITC stain within each individual nucleus and record in a table. Maybe there is a plugin to do this and I’m doing it the hard way, I just don’t know. Please help if you know how to do this. After having to upload the images as .TIFF, I’m not sure this will work for anyone to try in FIJI. Thank you in advance.
advpub_0609010004.pdf - Code is after the references
New Text Document (2).txt - Where I’m at with the code from the publication above