Asking help to calculate the length of cell by using plugin

1x10-6 M.tif (4.2 MB)

Please help me how to calculate the length of molecule by using plugin in Image J… because when I am using treshold, not all my molecule is detected. thank you for helping

Hi Tania,
Which molecule do you want to measure. It is very do-able if we know what to look for.

thank you for reply my message. please help me, how I can measure the length the aggregate with automatically. I already used plugin with ridge detection by fiji software. but, the result is not good, because some of circle is accounted in image which is I dont need it. please help me. I feel frustated now1x10-6 M.tif (4.2 MB) . thank you so much.