I have been working on analyzing brain tissue by tagging certain proteins and drawing area ROIs with the polygon selection tool to extract mean intensity values. Now I would like to turn the area ROIs (they are the shape of the letter “C”) into best fit curves and get a tangent plot profile along the best fit curve. I have attached an image so it is more easily understood.
I have found some ways I could approach the best fit curve and plot profile creation but I have not been able to find anything on turning an area selection to line selection.
I am trying to take the pixel intensity measurements of the “C” shaped object as it has 3 layers along that “C” shape as shown in figure 1.
If there is a better way to do this please let me know.