In cellprofiler I am trying to work out how much of GFP positive cells have infiltrated into a spheroid.
I have successfully segmented and identified GFP cells, spheroid and background as seperate objects. I just want to know if there is a way to work out the area of GFP object outside the spheroid in ‘background’ and then what’s the area of the GFP inside the spheroid object.
I basically want a similar sort of flow as used for Image Intensity where you can measure the intensity of image within an object but looking at area occupied.
Any help at all would be appreciated!