Area and shape measurement in SEM microscope images

Hi everybody,
I am new to image calculations, so I’ll start saying what I am trying to achieve so you can have a clear idea on how I want to proceed to measure an image. I want to calculate shape and size of different objects (insect omatidia) in SEM microscope images, I want to measure number and size of each ommatidia I was thinking of using the particle counter plugin incorporated in ImageJ but some of the regions of my eye are more shadier than others, so thresholding the image becomes complicated, this may be a recurrent question so if you can provide any reference answering a similar question I’ll appreciate it.

Thanks in advance.


Hello there Yesica,
It would help if you could upload a few images highlighting what you are planning to measure. It would then be easier to come up with a solution.

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