Good afternoon people! I need help with ImageJ.
I would like to measure the area and perimeter of intestinal microvilli (structures that are in the images). I measured microvilli in two ways and got different results.
In the first, I used straight and adjusted by the line width command to cover the microvilli shape (image 1). In the second, I used freehand selections to get around microvilli (image 2).
Looking at the results (image 3), I obtained a small difference for the area, but for the perimeter using freehand selections, the value was much higher.
Could someone tell me what is the most correct way to measure these two parameters?
I thank the attention!