I am trying to quantify the distribution of organelles (GFP+) inside the cells. The cells are labelled with DAPI.
I am trying to see if the distribution of the organelles changes between the WT and a KO cell line.
I am fairly new to Cellprofiler, so thank you for any help and suggestions!
After a lot of trying on Cell Profiler, I cannot find something equivalent to what was described in this discussion from 2014. Could you explain to me a way to measure an object radial distribution inside a cell? Is there something I missed?
I attached a copy of my cell profiler project with an example image to illustrate what I am talking about.
Thank you very much for any help you can give me!
LSavy_Radial distribution Lysosomes.cpproj (777.3 KB)