I try to quantify a chromogenic multiplex staining CD4-FoxP3 (Hematox, Purple membrane and DAB nucleus). I already did it with Fiji, but now I would like to use QuPath.
I already set up the stain vectors, I detected cells and managed to detect the purple CD4+ cells. But I’m stuck with the (CD4+)/FoxP3+ cells. I tried the single measurement classifier, the train object classifier, I tried to play with the deconvolution vectors, but I didn’t managed to get a good result.
The only good thing I get is when I use the pixel classifification/Create thresholder and use the Classify option. My FoxP3+ cells are perfectly classified.
But unfortunatelly, the classifyDetectionsByCentroid function erases all previously classifications made. Is there a trick to avoid this (ie to run this function only on selected detections ?) ?
Thanks a lot