I’m hoping I can get a little direction in the best way / most practical way to do this; I am trying to apply both Otsu’s and then Yen thresholding to an image stack.
But applying a threshold to the stack converts it to Black White so after that no more can be applied.
I also realised that I cannot apply these as a mask as the mask tool is done using single slice at a time.
Other than looping through each slice at a time and applying this. I am wondering is this the smartest way to do this, or is there an obvious solution I’m missing.
If anyone is wondering, I need Otsu to remove background noise from some 3D scanned oats, and then Yen allows me to filter out the shells and to see the seeds inside. Using only one creates problems that distort the image. For me.