Anyone uses LAP tracking method for RPE cells?

Hi I am working on the RPE cells and I want to track the mitosis events. The first idea that occurred to me is the LAP tracking method in cellprofiler. However, I found that it is really hard to optimize the parameters. Anyone who uses it can share his/her pipeline with me? Thank you very much

Best regards

Shirley

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It might be useful to engage the TrackMate community on this topic.

You might be interested in a few previous topics:

@bcimini and @tinevez might be able to assist further if you could give some more concrete and specific examples of the problems you are encountering.

Thanks for the reply! I will give it a try