Analyzying ROI within stack of images

Hey all,

I have a few movies of cells of bacteria containing rhodopsin being photobleached and want to take the intensity of one rhodopsin over time. But whenever I select and ROI the particle analyzer still just used the whole frame.

Add a selection to the ROI manager (press ‘t’ or use Edit->Selection->Add to Manager). In the ROI Manager menu select the submenu “More -> Multi Measure” to measure all slices without the Analyze Particles function (which requires a binary or thresholded image).

See also the ROI Manager documentation.

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Hi Natalie, I haven’t seen that happen before. The particle analyser always analysis the ROI you have drawn. Are you sure you have thresholded you images before using the particle analyser?