Analyzing the Making Projection Image

Hi,

I just created a pipeline to make projections from a stack of a time-lapse of moving cells which worked perfectly, but now I am wondering how I can analyze the image that I have created? I am looking for numerical values and/ or statistics on how the cells moved for the projection. I am not sure where to start for this problem.

Thank you!

Hi,

Can you give us some example images to understand better what you aim for?

Also, it’ll be great if you can share your experience with this topic : Image naming format for uploading

Thanks.

Are you saying you made a projection across time points? I’m not aware of any way to extract movement information if the time points have been projected into a single image- a better thing to do would be to use TrackObjects on your unprojected time points.

If that’s not the situation, would you mind clarifying?