Hello. I am a new (and completely inexperienced) user of cell profiler. I learned about the software from a publication by Cataldo et al, 2010 and I have to say that it sounds like a really helpful tool. I am interested on using the software to measure mitochondrial distribution around the nucleus, like they did in the publication. After I did some research, I found out that the pipeline they used in this publication has been uploaded in the forum but I have to admit that I encountered some difficulties while I was trying to analyze my images, since I am getting an error in the beginning of each analysis (see attached image). Therefore, I was wondering whether it is possible to get some step by step help on the issue. If someone can help me I will really appreciate it.
Thank you in advance for your time.