Analyzing dendritic spine motility



Hi All,

I am trying to determine the motility of dendritic protrusions and spines. (Basically track dendritic spines.) My time-lapse images were captured with Airyscan and I have maximum intensity projections. Unfortunately I encounter visible photobleaching due to the longer acquisition time, regardless of using very low laser power. Also, spines by nature keep appearing and disappearing. Any help would be greatly appreciated, I am still very new to image analysis. Attached are two frames of an example image.