I am interested in some imaging processing and analysis of data I have been collecting. I would like to get some statistics on the intensity of the cells in this grid.
Some processing steps would include:
-straighten and crop the array found in the center of the images below.
-apply a grid with a predefined number of rows and columns over the image such that each cell is indexed to the grid
-analyze each cell location and get statistics about brightness (the majority of cells are not emitting)
-determine the population of cells that are emitting
Apply this process over a set of images.
I am agnostic as to which software to use to do this. Please let me know your thoughts on how to get started with this.