Hi image J community,
I was wondering is it possible to use analyze particles tool with images that are not thresholded. I need to measure average pixel values in cells after background subtraction/masking and want to know to exact grey values in the ROIs. There are hundreds of cells in the image and analyze particle is the ideal tool for cell counting and finding out area of cells but I can’t retain the exact grey values within each cell that I would love to also get from “analyze particles” tool. Any help/suggestion would be really appreciated.