I’m trying to analyze the particles (black on white background), however I couldn’t include the particles inside the holes.
The raw image to be analyzed:
Result after using analyze particles:
Then, I tried using the morphology plugin: http://www.mecourse.com/landinig/software/software.html and by using the Numberparticles8 option I could select all the particles inside the holes. However, my problem is that it only showed the numbered results and not ROI, I wanted to measure the properties in the “shape descriptor” in the measurement option, also to not select particles below 0.05 um^2, and possibly have the result as ROI set.
Result after using numberparticles8 of morphology plugin:
Therefore is it possible to convert the results of Numberparticles8 into ROI so that I could measure it using the imagej measure option, or is it possible to include the particles inside the holes by modifying analyze particles?