I am trying to analyse the size and signal intensity of particles (dots) in the nucleus and cytoplasm of cells. Each image contains multiple cells, so I started to define ROIs identifying each cell and each nuclei. Then, I “thresholded” the image and proceeded to the Analyse Particle menu… I ended up generating hundreds of additional ROIs (for each dot in the entire image)… but I cannot identify the ones present in each cell/nucleus.
Is there a way to assign the analyse particle tool to a specific ROI?
I thank you in advance for your help,
All the best