Analyze Particles giving error on ImageJ


Hi Bob,
Thank you for your email. How can i manually set this threshold on imagej ?



Good day to you,
Just go to Edit > Adjust > Threshold and set the sliders or use Auto. It is best if you start with Auto
If you are not certain of the deeded values.
Good luck,


Ok, I am aware of setting threshold through this process. I thought there was a way to manually assign values to a region on the image. Thank you for all the help!


I get a count of 898 using the Process>Find Maxima command.


That’s great wayne, thanks a lot. Seems like this is another way to calculate colonies.