Today we have run into a problem with Analyze Particles when trying to use it within a macro, on different computers than we have in the past.
The problem is that on two computers particles are correctly reduced and counted. On two other computers only particles associated with the edge of the ROI are retained. This also occurs when Analyze Particles is used independently of the macro.
The problem is completely repeatable on all computers. All computers are running the same version of Windows and of ImageJ (1.52k). All used exactly the same files, ROIs, and Analyze Particles settings:
Analyze Particles settings: